May 24, 2012 biochemical activities in different accession of pongamia pinnata l. Plant tissue culture offers new ways for the improvement of this crop after many years of recalcitrance. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. Pongamia is a medium size, nitrogen fixing tree native to india, indonesia, malaysia, and myanmar. Morphological and molecular level of genetic diversity among. Clonal propagation of pongamia pinnata pierre through stem cuttings 103 clonal propagation of pongamia. It even has the potential to expand itself in the sun protected areas as well. Search from 60 top pinnata pictures and royaltyfree images from istock. How to merge pdfs and combine pdf files adobe acrobat dc. Fruit is brown in color and seed pods are poisonous. It is essential that the surface of the explant is sterilised to remove all microbial contamination. Pongamia or pongamia pinnata is an average sized tree. Peter 4 1department of botany, providence womens college, calicut 673009, kerala, india 2 project coordinator, all india coordinated research project on spices, indian institute of. Hplc analysis was performed on a c 18 column using an 85.
Studies and research in applied zoology, school of biological sciences, kuvempu university, karnataka, india. Micropropagation definition, the propagation of plants from tissue cultures, often from single cells. A rapid, simple and specific reversedphase hplc method has been developed for analysis of karanjin in pongamia pinnata linn. Pharmacognostic and phytochemical investigation of pongamia. Successfully been grown and conserved by micropropagation because of. Pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub. Evaluation of antibacterial activity ofpongamia pinnata l. It is found mainly in the native western ghats in india, northern australia, fiji and in some regions of eastern asia. Abstractbibliographic data and the results of present domestic experiments show that paulownia species can be grown easily, have high biomass production, favourable energetic parameters and modest requirements as to site quality. Analysis of genetic diversity in pongamiapongamia pinnata l. List of various diseases cured by pongamia pinnata. Explants carrying performed vegetative bud suitable for enhanced axillary branching. Soda pdf merge tool allows you to combine two or more documents into a single pdf file for free.
Road, avenue road cross, bangalore 560 076, india 2arid forest research institute icfre, new pali road, jodhpur 342 008, india. Our study was to screen the antibacterial activity of the seed extracts of pongamia pinnata linn. Karanja cake activated carbon as an adsorbent is that it facilitates complete utilisation of pongamia pinnata besides contributing to waste management. Aseptic method of clonal propagation is called as micropropagation and it offer the advantage of large number of truetotype plantlets can be produced with relatively short time and space from a single individual. Names of pongamia pinnata in various languages of the world are also given. Biochemical activities in different accession of pongamia. Medicinal uses, phytochemistry and pharmacology of pongamia. Pongamia millettia pinnata syn ponamia pinnata summary pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub with a broad distribution from india, through central and southeastern asia, indonesia and into northern australia. Antimicrobial activity of different extracts of pongamia pinnata. Micropropagation is the asexual propagation of plants from growing tissue be it whole. Micropropagation and mediumterm conservation of rosa. Peter 4 1department of botany, providence womens college, calicut 673009, kerala, india 2 project coordinator, all india coordinated research. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
Reactiveextraction of pongamia seeds for biodiesel production. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. Pongamia pinnata click image to enlarge photographs by. Development and validation of an hplc method for karanjin in. Medium sized tree growing up to 40 ft with a large spreading canopy up to 40 ft. Department of botany, government vidarbha institute of science and humanities, amravati444604, india. The study showed that in general, using activated carbons for biodiesel purification resulted in higher yields and better fuel properties. This free online tool allows to combine multiple pdf or image files into a single pdf document. Which has been found to consist of five sugars viz dmannose, dglucose, dxylose, raffinose, lfructose along with five. Chemical examination of the seeds of pongamia pinnata l. Plant cell division is slow compared to the growth of bacteria and fungi, and even minor contaminants will easily overgrow the plant tissue culture. The place having rainfall of 5002500 cm together with a temperature range of 1040 degree celsius is suitable for growing pongamia trees. It is the fact that micropropagation is the only commercially viable method of clonal propagation of most of the horticultural crops. Preparation of biodiesel from crude oil of pongamia pinnata.
Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Leaves of pongamia pinnata are digestive, laxative, antihelmintic and are good for diarrhea, leprosy. Pierre leguminaceae, subfamily papilionoideae is a medium sized tree commonly called as karanja that generally attains a height of about 1020 m and a trunk diameter of more than 50 cm pongamia. Biochemical activities in different accession of pongamia pinnata l. Aug 18, 2007 murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Micropropagation definition of micropropagation by the free.
The pongamia seeds were crushed in retsch model sm100 cutting mill hann, germany with feed rate up to 10kgh, feed size of pongamia pinnata l. It is antihelmintic and useful in beriberi, ophthalmology, dermatopathy, vaginopathy, and ulcers. Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. Analysis of genetic diversity in pongamia pongamia pinnata l pierrre using aflp markers mahendar thudi 1, revathi manthena, suhas p wani, leela tatikonda 1,2, david a hoisington 1 and rajeev k varshney1,3, 1international crops research institute for the semiarid tropics, patancheru, greater hyderabad 502 324, andhra pradesh, india. Pdf merge combinejoin pdf files online for free soda pdf. Native to tropical and subtropical areas, the pongam tree, or pongamia pinnata, was first introduced to hawaii in the 1860s. An efficient protocol for micropropagation and genetic. Micropropagation and mediumterm conservation of rosa pulverulenta somayeh kavand, maryam jafarkhani kermani, ali haghnazari, pegah khosravi and mohamad reza azimi tissue culture and gene transformation department, agricultural biotechnology research institute of iran,islamic republic of iran. Micropropagation of pongamia pinnata through enhanced. Antimicrobial activity of different extracts of pongamia pinnata p. The in vivo clonal propagation is often difficult, expensive and even unsuccessful. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Plant details information about pongamia pinnata plant. Deze gratis online tool maakt het mogelijk om meerdere pdf bestanden of afbeeldingen te combineren in een pdf document.
Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. Uses, benefits, cures, side effects, nutrients in pongamia pinnata. Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. Pierre accessions s p ahlawat1, r v kumar 2, rajeev ranjan 1, s k pandey, d c joshi and s k dhyani 1icarcentral agroforestry research institute, jhansi 284 002, india 2icarindian grassland and fodder research institute, jhansi 284 003. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. Millettia pinnata is a species of tree in the pea family, fabaceae, native to eastern and tropical asia, australia and pacific islands. This tree expands perfectly in areas having excellent daylight. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits. The present paper deals with chemical examination of defatted seeds of the plant pongamia pinnata l. Pongamia pinnata, floral phenology, pod maturation, fruit colours, seed and seedling quality characters. The fresh bark of pongamia pinnata is sweet and mucilaginous to taste, soon become bitter and acrid. The method was validated for accuracy, precision, linearity. Use of the wood is limited to cabinetmaking, cartwheels. The major defining benefit of using pongamia as a biofuel feedstock.
In pongamia, no such undulations were noted in the axillary meristems after 4 wk of culture on tdz, but the swelling in the meristem was obvious. Aug 23, 20 the legume tree pongamia pinnata also called millettia pinnata is gaining importance as a biofuel feedstock tree because of the abundant annual production of oilrich seeds, adaptation to a wide range of geoclimatic conditions and significant resistance to abiotic stress, such as waterdeficit, salinity and acidity of soils. However, further study on the phytochemistry and the mechanisms of the chemical constituents in exhibiting certain biological activities are required to fully understand the phytochemical profile and the complex pharmacological effects. Reactiveextraction of pongamia seeds reactiveextraction of pongamia seeds was carried out using whole seeds with seed coat. Biotechnology for micropropagation and enhancing variations. It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. It is important to note that crude pongamia oil was used in this study for biodiesel preparation and since the degree of refinement of the vegetable oil affects the yield of ester formation freedman et al. An efficient protocol for micropropagation and genetic stability analysis of melia dubia cav. Virus free plant sub millimeter portion of shoottip stock plant virus free nodal cutting disadvantage of using small sized explant for micropropagation shoot tip explant low survival rate and slow initial growth. Application of tissue culture techniques for genetic up gradation of economically important plants has been reported. Pongamia pinnata herb uses, benefits, cures, side effects. Antimicrobial efficacy of some medicinal plant extract. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Preparation of biodiesel from crude oil of pongamia.
Evaluation of antibacterial activity of pongamia pinnata l. Pdf micropropagation of mature pongamia pinnata pierre. Micropropagation definition of micropropagation at. Evaluation of antibacterial activity of pongamia pinnata. This research work consists protein profiling and enzyme estimation of p. Micropropagation definition of micropropagation by the. The pongamia seeds were crushed in retsch model sm100 cutting mill hann, germany with feed rate up to 10kgh, feed size of of 0. These crushed seeds were separated by sieves to give. Pdf pongamia pinnata is gaining lot of importance as a potential oil yielding tree. Chapter2 micropropagation and large scale culture of c. How pongamia pinnata is effective for various diseases is listed in repertory format.
Pdf clonal propagation of pongamia pinnata pierre through. Biotechnology for micropropagation and enhancing variations in vanilla minoo divakaran 1, k. Pongamia pinnata pierre is a tree legume, having potential in production of raw material for biodiesel. Macropropagation of acacias authorstream presentation. Tissue culture is the in vitro aseptic culture of cells, tissues, organs or whole plant. Pongamia pinnata pongam environmental horticulture university. The wood is yellowish white, coarse, hard, and beautifully grained, but not durable. A protocol for in wk propagation of this plant was standardized using seedling explants. S department of agriculture was given seeds from sri lanka.
Plant tissue culture case study 3 demonstration of. Development and validation of an hplc method for karanjin. Combine pdfs in the order you want with the easiest pdf merger available. New tree species for agroforestry and energy purposes. Find highquality stock photos that you wont find anywhere else. Ps2pdf free online pdf merger allows faster merging of pdf files without a limit or watermark. Autotrophic plants, green algae, and red algae which generally acquire nutrients through photosynthesis and display alternation of generation. Pierre accessions s p ahlawat1, r v kumar 2, rajeev ranjan 1, s k pandey, d c joshi and s k dhyani 1icarcentral agroforestry research institute, jhansi 284 002, india 2icarindian grassland and fodder research institute, jhansi 284 003, india.
A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are. Easily combine multiple files into one pdf document. Due to recent interest in biofuels, pongamia is often the generic name used when referring to millettia pinnata, a tree being explored for producing biodiesel. Micropropagationomicsgroupjournal of biotechnology and. The parenchymatous cell that associated with the fibers was more thick walled and contained bigger clustertype calcium oxalate crystals. Jain 1, shashi alok 1, deepak kailasiya 1, vinod kr. In vitro micropropagation of yew taxus baccata and.
Morphological and molecular level of genetic diversity among pongamia pongamia pinnata l. The fibers and sclereids of the pedicel were similar to those found in the pods but were larger. Please visit our seed store to view current selections. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Principles of tissue cult ure and micropropagation. Nodulation in the legume biofuel feedstock tree pongamia pinnata. A brownish green or bluish black coloration that resulting with the addition of few drops 0. It has been successfully introduced to humid tropic lowland. Micropropagation of mature pongamia pinnata pierre springerlink. Micropropagation offers the potential to produce thousands or even millions of plants per annum. Pongamia pinnata a sustainable biofuel plant species for semi arid climatic.
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